ngc quest 10plus fast protein liquid chromatography fplc system Search Results


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Bio-Rad quest 10 plus ngc protein chromatography system
Quest 10 Plus Ngc Protein Chromatography System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ngc Quest 10 Plus Fplc System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad bio rad ngc quest 10 plus fast protein liquid chromatography apparatus
Bio Rad Ngc Quest 10 Plus Fast Protein Liquid Chromatography Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad anion exchange fast protein liquid chromatography fplc
Production of fully-humanized biologic miR-328-3p molecule. (A) Schematic illustration of the fully-humanized bioengineered RNA agent (hBERA) bearing the target miR-328-3p (hBERA/miR-328, 192 nucleotides in length). The carrier consists of a human serine tRNA (htRNA Ser ) and a modified pre-miR-34a 23 , in which miR-34a duplexes are replaced by target miR-328 sequences. (B) High-level heterogeneous expression of hBERA/miR-328 through bacterial fermentation, as demonstrated by urea-PAGE analysis. Total RNA from wild type (WT) Escherichia coli was used for comparison. (C) <t>FPLC</t> trace during the purification of hBERA/miR-328. The insert shows urea-PAGE analysis of target RNA fractions collected during FPLC separation. (D) The purity of isolated hBERA/miR-328-3p (98.5%) was determined by HPLC analysis. Au/Uv, absorbance unit of ultraviolet-visible spectroscopy; nt, nucleotide.
Anion Exchange Fast Protein Liquid Chromatography Fplc, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad size exclusion chromatography
Production of fully-humanized biologic miR-328-3p molecule. (A) Schematic illustration of the fully-humanized bioengineered RNA agent (hBERA) bearing the target miR-328-3p (hBERA/miR-328, 192 nucleotides in length). The carrier consists of a human serine tRNA (htRNA Ser ) and a modified pre-miR-34a 23 , in which miR-34a duplexes are replaced by target miR-328 sequences. (B) High-level heterogeneous expression of hBERA/miR-328 through bacterial fermentation, as demonstrated by urea-PAGE analysis. Total RNA from wild type (WT) Escherichia coli was used for comparison. (C) <t>FPLC</t> trace during the purification of hBERA/miR-328. The insert shows urea-PAGE analysis of target RNA fractions collected during FPLC separation. (D) The purity of isolated hBERA/miR-328-3p (98.5%) was determined by HPLC analysis. Au/Uv, absorbance unit of ultraviolet-visible spectroscopy; nt, nucleotide.
Size Exclusion Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DUTSCHER DOMINIQUE ngc quest 10 plus system
Production of fully-humanized biologic miR-328-3p molecule. (A) Schematic illustration of the fully-humanized bioengineered RNA agent (hBERA) bearing the target miR-328-3p (hBERA/miR-328, 192 nucleotides in length). The carrier consists of a human serine tRNA (htRNA Ser ) and a modified pre-miR-34a 23 , in which miR-34a duplexes are replaced by target miR-328 sequences. (B) High-level heterogeneous expression of hBERA/miR-328 through bacterial fermentation, as demonstrated by urea-PAGE analysis. Total RNA from wild type (WT) Escherichia coli was used for comparison. (C) <t>FPLC</t> trace during the purification of hBERA/miR-328. The insert shows urea-PAGE analysis of target RNA fractions collected during FPLC separation. (D) The purity of isolated hBERA/miR-328-3p (98.5%) was determined by HPLC analysis. Au/Uv, absorbance unit of ultraviolet-visible spectroscopy; nt, nucleotide.
Ngc Quest 10 Plus System, supplied by DUTSCHER DOMINIQUE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad protein a step
Production of fully-humanized biologic miR-328-3p molecule. (A) Schematic illustration of the fully-humanized bioengineered RNA agent (hBERA) bearing the target miR-328-3p (hBERA/miR-328, 192 nucleotides in length). The carrier consists of a human serine tRNA (htRNA Ser ) and a modified pre-miR-34a 23 , in which miR-34a duplexes are replaced by target miR-328 sequences. (B) High-level heterogeneous expression of hBERA/miR-328 through bacterial fermentation, as demonstrated by urea-PAGE analysis. Total RNA from wild type (WT) Escherichia coli was used for comparison. (C) <t>FPLC</t> trace during the purification of hBERA/miR-328. The insert shows urea-PAGE analysis of target RNA fractions collected during FPLC separation. (D) The purity of isolated hBERA/miR-328-3p (98.5%) was determined by HPLC analysis. Au/Uv, absorbance unit of ultraviolet-visible spectroscopy; nt, nucleotide.
Protein A Step, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad uno q6 anion exchange column
Production of fully-humanized biologic miR-328-3p molecule. (A) Schematic illustration of the fully-humanized bioengineered RNA agent (hBERA) bearing the target miR-328-3p (hBERA/miR-328, 192 nucleotides in length). The carrier consists of a human serine tRNA (htRNA Ser ) and a modified pre-miR-34a 23 , in which miR-34a duplexes are replaced by target miR-328 sequences. (B) High-level heterogeneous expression of hBERA/miR-328 through bacterial fermentation, as demonstrated by urea-PAGE analysis. Total RNA from wild type (WT) Escherichia coli was used for comparison. (C) <t>FPLC</t> trace during the purification of hBERA/miR-328. The insert shows urea-PAGE analysis of target RNA fractions collected during FPLC separation. (D) The purity of isolated hBERA/miR-328-3p (98.5%) was determined by HPLC analysis. Au/Uv, absorbance unit of ultraviolet-visible spectroscopy; nt, nucleotide.
Uno Q6 Anion Exchange Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad ion exchange column
Production of fully-humanized biologic miR-328-3p molecule. (A) Schematic illustration of the fully-humanized bioengineered RNA agent (hBERA) bearing the target miR-328-3p (hBERA/miR-328, 192 nucleotides in length). The carrier consists of a human serine tRNA (htRNA Ser ) and a modified pre-miR-34a 23 , in which miR-34a duplexes are replaced by target miR-328 sequences. (B) High-level heterogeneous expression of hBERA/miR-328 through bacterial fermentation, as demonstrated by urea-PAGE analysis. Total RNA from wild type (WT) Escherichia coli was used for comparison. (C) <t>FPLC</t> trace during the purification of hBERA/miR-328. The insert shows urea-PAGE analysis of target RNA fractions collected during FPLC separation. (D) The purity of isolated hBERA/miR-328-3p (98.5%) was determined by HPLC analysis. Au/Uv, absorbance unit of ultraviolet-visible spectroscopy; nt, nucleotide.
Ion Exchange Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA bugbuster® 1x
Production of fully-humanized biologic miR-328-3p molecule. (A) Schematic illustration of the fully-humanized bioengineered RNA agent (hBERA) bearing the target miR-328-3p (hBERA/miR-328, 192 nucleotides in length). The carrier consists of a human serine tRNA (htRNA Ser ) and a modified pre-miR-34a 23 , in which miR-34a duplexes are replaced by target miR-328 sequences. (B) High-level heterogeneous expression of hBERA/miR-328 through bacterial fermentation, as demonstrated by urea-PAGE analysis. Total RNA from wild type (WT) Escherichia coli was used for comparison. (C) <t>FPLC</t> trace during the purification of hBERA/miR-328. The insert shows urea-PAGE analysis of target RNA fractions collected during FPLC separation. (D) The purity of isolated hBERA/miR-328-3p (98.5%) was determined by HPLC analysis. Au/Uv, absorbance unit of ultraviolet-visible spectroscopy; nt, nucleotide.
Bugbuster® 1x, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad uno s6 column
Production of fully-humanized biologic miR-328-3p molecule. (A) Schematic illustration of the fully-humanized bioengineered RNA agent (hBERA) bearing the target miR-328-3p (hBERA/miR-328, 192 nucleotides in length). The carrier consists of a human serine tRNA (htRNA Ser ) and a modified pre-miR-34a 23 , in which miR-34a duplexes are replaced by target miR-328 sequences. (B) High-level heterogeneous expression of hBERA/miR-328 through bacterial fermentation, as demonstrated by urea-PAGE analysis. Total RNA from wild type (WT) Escherichia coli was used for comparison. (C) <t>FPLC</t> trace during the purification of hBERA/miR-328. The insert shows urea-PAGE analysis of target RNA fractions collected during FPLC separation. (D) The purity of isolated hBERA/miR-328-3p (98.5%) was determined by HPLC analysis. Au/Uv, absorbance unit of ultraviolet-visible spectroscopy; nt, nucleotide.
Uno S6 Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Production of fully-humanized biologic miR-328-3p molecule. (A) Schematic illustration of the fully-humanized bioengineered RNA agent (hBERA) bearing the target miR-328-3p (hBERA/miR-328, 192 nucleotides in length). The carrier consists of a human serine tRNA (htRNA Ser ) and a modified pre-miR-34a 23 , in which miR-34a duplexes are replaced by target miR-328 sequences. (B) High-level heterogeneous expression of hBERA/miR-328 through bacterial fermentation, as demonstrated by urea-PAGE analysis. Total RNA from wild type (WT) Escherichia coli was used for comparison. (C) FPLC trace during the purification of hBERA/miR-328. The insert shows urea-PAGE analysis of target RNA fractions collected during FPLC separation. (D) The purity of isolated hBERA/miR-328-3p (98.5%) was determined by HPLC analysis. Au/Uv, absorbance unit of ultraviolet-visible spectroscopy; nt, nucleotide.

Journal: Acta Pharmaceutica Sinica. B

Article Title: Bioengineered miR-328-3p modulates GLUT1-mediated glucose uptake and metabolism to exert synergistic antiproliferative effects with chemotherapeutics

doi: 10.1016/j.apsb.2019.11.001

Figure Lengend Snippet: Production of fully-humanized biologic miR-328-3p molecule. (A) Schematic illustration of the fully-humanized bioengineered RNA agent (hBERA) bearing the target miR-328-3p (hBERA/miR-328, 192 nucleotides in length). The carrier consists of a human serine tRNA (htRNA Ser ) and a modified pre-miR-34a 23 , in which miR-34a duplexes are replaced by target miR-328 sequences. (B) High-level heterogeneous expression of hBERA/miR-328 through bacterial fermentation, as demonstrated by urea-PAGE analysis. Total RNA from wild type (WT) Escherichia coli was used for comparison. (C) FPLC trace during the purification of hBERA/miR-328. The insert shows urea-PAGE analysis of target RNA fractions collected during FPLC separation. (D) The purity of isolated hBERA/miR-328-3p (98.5%) was determined by HPLC analysis. Au/Uv, absorbance unit of ultraviolet-visible spectroscopy; nt, nucleotide.

Article Snippet: Purification of target RNA molecules was achieved by using the anion exchange fast protein liquid chromatography (FPLC) methods , on an NGC QUEST 10PLUS FPLC system (Bio-Rad).

Techniques: Modification, Expressing, Comparison, Purification, Isolation, Spectroscopy